The article discusses the use of Fluorescent In Situ Hybridization (FISH) as a powerful tool for studying biodeterioration in cultural heritage (CH). Specifically, it highlights the importance of detecting and identifying biodeteriogenic microorganisms, such as Paenibacillus spp., which pose a threat to the preservation of CH objects.
The FISH technique allows for in situ analysis, enabling the rapid and specific identification of individual microbial cells without the need for culture isolation. By utilising probes like Pab489 designed for Paenibacillus sp., researchers can efficiently detect and identify target microorganisms associated with the deterioration of CH assets.
Furthermore, the study emphasises the potential of FISH methodology in the field of CH preservation by offering a fast and culture-independent approach to studying microbial communities. The use of flow cytometry and epifluorescence microscopy with specific probes like Pab489-Cy3 demonstrates high efficiency and specificity in detecting Paenibacillus sp., a common biodeteriogenic agent. This methodology not only aids in the identification of microbial threats to CH but also opens avenues for exploring the biotechnological or medical potential of Paenibacillus strains found in these environments.
Overall, the research underscores the significance of FISH in advancing our understanding of microbial communities associated with the biodeterioration of cultural heritage. By providing a rapid and accurate means of detecting and identifying target microorganisms, FISH contributes to the safeguarding and preservation of CH assets, offering insights into potential strategies for mitigating biodeterioration risks and exploring the biotechnological applications of microorganisms in cultural heritage contexts.
