This study focuses on the analysis of Edvard Munch’s paintings to develop conservation strategies by investigating the microbial communities colonising the artworks. The research employed high-throughput sequencing techniques to characterise the prokaryotic community present on selected sketches showing signs of deterioration due to biocolonisation.
By using Illumina-based deep sequencing on 16S rDNA amplicons, the study aimed to understand the distribution patterns of microbiota on different sketches with similar deterioration signs. The analysis revealed the presence of proteolytic bacteria in the microsamples, indicating their potential role in the degradation of proteins present in the paint layers.
Furthermore, the study detected casein as the binder used by the artist, highlighting the importance of identifying materials to guide conservation and restoration processes. Immunoenzymatic assays were conducted to detect casein and explore the microbial communities thriving on the artworks. The findings suggest that the bacterial communities found in the paintings consist mainly of species with proteolytic capacity, which could contribute to the deterioration observed in the artworks.
Overall, this research sheds light on the microbial diversity present on colonising communities in artworks, providing valuable insights for conservation interventions in cultural heritage assets. By understanding the materials used by the artist, identifying the dynamics of colonising microbiota, and detecting proteolytic bacteria, this study contributes to the development of effective conservation strategies to preserve Edvard Munch’s paintings and other cultural heritage artworks.
